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  • 产品名称:anti-STX8antibody(Syntaxin8)(AA1-215)

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简单介绍:
anti-STX8antibody(Syntaxin8)(AA1-215)
详情介绍:
Immunogen Recombinant cytoplasmic domain of rat syntaxin 8 (aa 1-215).
Specificity Specific for syntaxin 8.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Syntaxin 8 (STX8 Antibody Abstract)
Research Area Signaling, Organelles, Chromatin and Nuclear Signaling, Cytoskeleton, Microfilaments
Pathways Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 100 up to 1 : 5000 (AP staining)
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Syntaxin 8 (STX8) (AA 1-215), (Cytoplasmic Domain) antibody (ABIN1742226) dilution: 1 : 1000, sample: crude synaptic membranes fraction of rat brain (LP1)
Immunocytochemistry (ICC) image for anti-Syntaxin 8 (STX8) (AA 1-215), (Cytoplasmic Domain) antibody (ABIN1742226) Indirect immunostaining of Hela cells with anti-syntaxin 8 (dilution 1 : 100; red) an...
Product cited in: Pattu, Qu, Schwarz, Strauss, Weins, Bhat, Halimani, Marshall, Rettig, Hoth: "SNARE protein expression and localization in human cytotoxic T lymphocytes." in: European journal of immunology, Vol. 42, Issue 2, pp. 470-5, 2012 (PubMed).

Madan, Rastogi, Parashuraman, Mukhopadhyay: "Salmonella acquires lysosome-associated membrane protein 1 (LAMP1) on phagosomes from Golgi via SipC protein-mediated recruitment of host Syntaxin6." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5574-87, 2012 (PubMed).

Offenh?user, Lei, Roy, Collins, Stow, Murray: "Syntaxin 11 binds Vti1b and regulates late endosome to lysosome fusion in macrophages." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 6, pp. 762-73, 2011 (PubMed).

Flowerdew, Burgoyne: "A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels." in: The Biochemical journal, Vol. 418, Issue 3, pp. 529-40, 2009 (PubMed).

Takamori, Holt, Stenius, Lemke, Gr?nborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gr?ter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, 2006 (PubMed).

Murray, Wylie, Khromykh, Hume, Stow: "Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10478-83, 2005 (PubMed).

Nakamura, Fukuda, Kato, Hirose: "MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking." in: Molecular biology of the cell, Vol. 16, Issue 4, pp. 1696-710, 2005 (PubMed).