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  • 产品名称：anti-GABRB3antibody(gamma-aminobutyricAcid(GABA)AReceptor,beta3)(AA344-429)

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简单介绍：
anti-GABRB3antibody(gamma-aminobutyricAcid(GABA)AReceptor,beta3)(AA344-429)
详情介绍：
Immunogen Recombinant protein (aa 344-429 in mouse) of the unprocessed precursor forced into a loop-shaped structure.
Specificity Specific for GABA-A receptor beta3.
Purification antiserum
Alternative Name GABA-A Receptor Beta3 (GABRB3 Antibody Abstract)
Background Synonyms: GABA(A) R beta3
Pathways Sensory Perception of Sound
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500
Comment

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3) (AA 344-429) antibody (ABIN1742465) dilution: 1 : 1000, sample: unboiled synaptic membrane fraction of rat brain (LP1)
Immunocytochemistry (ICC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3) (AA 344-429) antibody (ABIN1742465) Indirect immunostaining of PFA fixed mouse cortex neurons (dilution 1 : 500)
Immunohistochemistry (IHC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3) (AA 344-429) antibody (ABIN1742465) Indirect immunostaining of a rat hippocampus section fixed with 4 % PFA and 15 % picr...
Product cited in: Kerti-Szigeti, Nusser, Eyre: "Synaptic GABAA receptor clustering without the ?2 subunit." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 31, pp. 10219-33, 2014 (PubMed).