Comment
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WB: The crude antiserum is highly recommended. This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC/IHC: The affinity purified antibody is highly recommended for these applications. For best results use the protocol of Schneider Gasser et al., 2006. ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
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