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  • 产品名称：anti-GABRA2antibody(gamma-aminobutyricAcid(GABA)AReceptor,alpha2)(AA29-37)

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简单介绍：
anti-GABRA2antibody(gamma-aminobutyricAcid(GABA)AReceptor,alpha2)(AA29-37)
详情介绍：
Immunogen Synthetic peptide (aa 29-37 of rat GABA-A receptor alpha2 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Specificity Specific for GABA-A receptor alpha2.
Purification antiserum
Alternative Name GABA-A Receptor Alpha2 (GABRA2 Antibody Abstract)
Background Synonyms: GABA(A) R alpha2
Application Notes WB: 1 : 1000 (AP staining)
ICC: not recommended
IHC: not recommended
Comment

WB: The crude antiserum is highly recommended. This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC/IHC: The affinity purified antibody is highly recommended for these applications. For best results use the protocol of Schneider Gasser et al., 2006. ICC: This antibody is suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 2 (GABRA2) (AA 29-37) antibody (ABIN1742453) dilution: 1 : 1000, sample: unboiled synaptic membrane fraction of rat brain (LP1)
Product cited in: Ardi, Albrecht, Richter-Levin, Saha, Richter-Levin: "Behavioral profiling as a translational approach in an animal model of posttraumatic stress disorder." in: Neurobiology of disease, Vol. 88, pp. 139-47, 2016 (PubMed).