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产品名称:anti-Synaptopodin(SYNPO)(AA331-452),(Isoform2)antibody
产品型号:
产品厂商:SYSY
产品文档:
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简单介绍:
anti-Synaptopodin(SYNPO)(AA331-452),(Isoform2)antibody
详情介绍:
Product details
Product details
Immunogen
Recombinant mouse synaptopodin (aa 331-452 in isoform 2).
Specificity
Fragment used for immunization is present in all 3 isoforms.
Purification
antiserum
Target details
Target details
Alternative Name
Synaptopodin (SYNPO
Antibody
Abstract)
Research Area
Neurology
Application Details
Application Details
Application Notes
WB: 1 : 500 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 200
IHC-P: 1 : 200
Comment
WB: Application is limited, the antibody is less sensitive than the rabbit antibody.
Restrictions
For Research Use only
Handling
Handling
Format
Liquid
Buffer
PBS
Handling Advice
Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage
4 °C/-20 °C
Storage Comment
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Images
Images
Supplier Images
Indirect immunofluorescence labeling of cultured hippocampal neurons with anti-synapt...
Immunostaining of a glomerolus in a mouse kidney section (dilution 1 : 200). Immunore...
Immunostaining of a paraffin embedded mouse kidney section (dilution 1 : 200; red). N...
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Images for product: anti-Synaptopodin (SYNPO) (AA 331-452), (Isoform 2) antibody
Indirect immunofluorescence labeling of cultured hippocampal neurons with anti-synaptopodin (dilution 1 : 500; red) and anti-synapsin (cat. no. 106 001; dilution 1 : 500; green).
Immunostaining of a glomerolus in a mouse kidney section (dilution 1 : 200). Immunoreactivity was revealed using a Cy3 conjugated secondary antibody. Nuclei were visualized by DAPI staining (blue).
Immunostaining of a paraffin embedded mouse kidney section (dilution 1 : 200; red). Nuclei were visualized by DAPI staining (blue).
References
References
Product cited in:
Wang, Dumoulin, Renner, Triller, Specht: "The Role of Synaptopodin in Membrane Protein Diffusion in the Dendritic Spine Neck."
in:
PLoS ONE
, Vol. 11, Issue 2, pp. e0148310, 2016 (PubMed).