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  • 产品名称:anti-NSFantibody(N-Ethylmaleimide-SensitiveFactor)(AA733-744)

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  • 产品厂商:SYSY
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简单介绍:
anti-NSFantibody(N-Ethylmaleimide-SensitiveFactor)(AA733-744)
详情介绍:
Immunogen Synthetic peptide REEGASPLDFD (aa 733-744 in rat NSF) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for NSF.
Purification antiserum
Alternative Name NSF (NSF Antibody Abstract)
Research Area Signaling, Metabolism
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 1000
IHC: 1 : 200
Comment

ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742269 as capture antibody (protocol for sandwich-ELISA).

Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 733-744) antibody (ABIN1742270) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 733-744) antibody (ABIN1742270) Indirect immunostaining of hippocampus neurons (dilution 1 : 500)
Product cited in: de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, 2015 (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kr?hnert, Sch?fer, Rammner, Koo, Cla?en, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, 2014 (PubMed).

Tian, Wu, Unzicker, Lu, Cai, Li, Schirra, Matti, Stevens, Deng, Rettig, Sheng: "The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 45, pp. 10546-55, 2005 (PubMed).

Tailleux, Neyrolles, Honoré-Bouakline, Perret, Sanchez, Abastado, Lagrange, Gluckman, Rosenzwajg, Herrmann: "Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 1939-48, 2003 (PubMed).

Michaut, Tomes, De Blas, Yunes, Mayorga: "Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 18, pp. 9996-10001, 2000 (PubMed).