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  • 产品名称：anti-GAD65antibody(GlutamateDecarboxylase2(PancreaticIsletsandBrain,65kDa))(AA3

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  • 产品厂商：SYSY
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简单介绍：
anti-GAD65antibody(GlutamateDecarboxylase2(PancreaticIsletsandBrain,65kDa))(AA3-96)
详情介绍：
Immunogen Purified recombinant mouse GAD 2 (aa 3-96).
Specificity Specific for GAD 2 / GAD 65.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name GAD 2 (GAD2 Antibody Abstract)
Background Synonyms: GAD 65
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 2000
IHC: 1 : 1000
Comment

ICC/IHC: The crude antiserum is recommended. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742417 as capture antibody (protocol for sandwich-ELISA).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 3-96) antibody (ABIN1742419) dilution: 1 : 1000, sample: synaptic vesicle fraction of rat brain (LP2)
Immunocytochemistry (ICC) image for anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 3-96) antibody (ABIN1742419) Indirect immunolabeling of PFA fixed cultured rat hippocampus neurons (dilution 1 : 500)
Product cited in: Du, Chazalon, Bestaven, Leste-Lasserre, Baufreton, Cazalets, Cho, Garret: "Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease." in: Neuroscience, Vol. 329, pp. 363-79, 2016 (PubMed).

Saliba, Gu, Yan, Moss: "Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition." in: The Journal of biological chemistry, Vol. 284, Issue 47, pp. 32544-50, 2009 (PubMed).