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  • 产品名称:anti-GABRA6antibody(gamma-aminobutyricAcid(GABA)AReceptor,alpha6)(AA20-30)

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  • 产品厂商:SYSY
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简单介绍:
anti-GABRA6antibody(gamma-aminobutyricAcid(GABA)AReceptor,alpha6)(AA20-30)
详情介绍:
Immunogen Synthetic peptide (aa 20-30 of rat GABA-A receptor alpha6 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Specificity Specific for GABA-A receptor alpha6.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name GABA-A Receptor Alpha6 (GABRA6 Antibody Abstract)
Background Synonyms: GABA(A) R alpha6
Pathways Synaptic Membrane
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500
Comment

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6) (AA 20-30) antibody (ABIN1742463) dilution: 1 : 1000, sample: unboiled rat cerebellum lysate
Immunohistochemistry (IHC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6) (AA 20-30) antibody (ABIN1742463) Indirect immunostaining of PFA fixed mouse cerebellum sections (dilution 1 : 500).
Product cited in: Joensuu, Tegelberg, Reinmaa, Segerstr?le, Hakala, Pehkonen, Korpi, Tyynel?, Taira, Hovatta, Kopra, Lehesjoki: "Gene expression alterations in the cerebellum and granule neurons of Cstb(-/-) mouse are associated with early synaptic changes and inflammation." in: PLoS ONE, Vol. 9, Issue 2, pp. e89321, 2014 (PubMed).