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  • 产品名称：anti-CACNA1Bantibody(CalciumChannel,Voltage-Dependent,NType,alpha1BSubunit)(AA2

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简单介绍：
anti-CACNA1Bantibody(CalciumChannel,Voltage-Dependent,NType,alpha1BSubunit)(AA2074-2314)
详情介绍：
Immunogen Recombinant protein (aa 2074-2314 of rat Ca2+-channel alpha-1B subunit).
Specificity Specific for Ca2+ channel alpha-1B.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Ca2+ Channel (CACNA1B Antibody Abstract)
Background Synonyms: Cav2.2
Application Notes WB: 1 : 1000
IP: not tested yet
ICC: not recommended
IHC: not recommended
Comment

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. WB: This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) (AA 2074-2314) antibody (ABIN1742327) anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) (AA 2074-2314) antibody
Product cited in: Lenkey, Kirizs, Holderith, Máté, Szabó, Vizi, Hájos, Nusser: "Tonic endocannabinoid-mediated modulation of GABA release is independent of the CB1 content of axon terminals." in: Nature communications, Vol. 6, pp. 6557, 2015 (PubMed).