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  • 产品名称：anti-Homer1/2/3(N-Term)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-Homer1/2/3(N-Term)antibody
详情介绍：
Immunogen Purified recombinant N-terminal parts of Homer 1, 2 and 3.
Specificity The first 120 aa were used for affinity purification. According to Soloviev et al. (2000), they are homologous in Homer 1 a-d, Homer 2 a-d, and Homer 3 a-d.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Background Synonyms: VesL 1, Syn47
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet
ELISA: not tested yet
Comment

ICC: PFA fixation recommended. Methanol fixation leads to a complete loss of the signal.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Homer 1/2/3 (N-Term) antibody (ABIN1742340) dilution: 1 : 1000, sample: rat brain homogenate
Immunocytochemistry (ICC) image for anti-Homer 1/2/3 (N-Term) antibody (ABIN1742340) Indirect immunolabeling of cultured hippocampus neurons with anti-Homer 1/2/3 (diluti...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Homer 1/2/3 (N-Term) antibody (ABIN1742340) Indirect immunostaining of PFA fixed paraffin embedded mouse cortex section with anti...
Product cited in: Tao-Cheng, Thein, Yang, Reese, Gallant: "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, 2014 (PubMed).

Tronson, Guzman, Guedea, Huh, Gao, Schwarz, Radulovic: "Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear." in: Biological psychiatry, Vol. 68, Issue 11, pp. 1007-15, 2010 (PubMed).