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  • 产品名称:anti-GlutamateReceptor,Ionotropic,Kainate5(GRIK5)(AA828-979),(C-Term)antibody

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  • 产品厂商:SYSY
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简单介绍:
anti-GlutamateReceptor,Ionotropic,Kainate5(GRIK5)(AA828-979),(C-Term)antibody
详情介绍:
Immunogen Recombinant protein of rat GluK 5 (C-terminus, aa 828-979).
Specificity Specific for GluK 5.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name KA 2 (GRIK5 Antibody Abstract)
Background Synonyms: GluR-gamma2
Pathways Carbohydrate Homeostasis, Synaptic Membrane, Maintenance of Protein Location, Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000
Comment

IHC: Antibody requires Clarke liquid fixation (1 vol. acetic acid, 6 vol. ethanol, 15 min at RT).

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Glutamate Receptor, Ionotropic, Kainate 5 (GRIK5) (AA 828-979), (C-Term) antibody (ABIN1742375) dilution: 1 : 1000, sample: synaptic membrane fraction of rat brain (LP1)
Immunohistochemistry (IHC) image for anti-Glutamate Receptor, Ionotropic, Kainate 5 (GRIK5) (AA 828-979), (C-Term) antibody (ABIN1742375) Indirect immunohystochemistry of a mouse hippocampus cryo-section (dilution 1 : 1000).
Product cited in: Fujikawa, Petralia, Fitzgerald, Wang, Millis, Morgado-Díaz, Kitamura, Kachar: "Localization of kainate receptors in inner and outer hair cell synapses." in: Hearing research, Vol. 314, pp. 20-32, 2014 (PubMed).

Marrocco, Mairesse, Ngomba, Silletti, Van Camp, Bouwalerh, Summa, Pittaluga, Nicoletti, Maccari, Morley-Fletcher: "Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 48, pp. 17143-54, 2012 (PubMed).