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  • 产品名称:anti-RegulatingSynapticMembraneExocytosis1(RIMS1)(AA207-366)antibody

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简单介绍:
anti-RegulatingSynapticMembraneExocytosis1(RIMS1)(AA207-366)antibody
详情介绍:
Immunogen Recombinant protein (aa 207-366) of rat RIM 1, a stretch of divergent aa connecting the Rab binding motif and the PDZ domain.
Specificity Specific for RIM 1
Cross-Reactivity (Details) no cross reactivity to RIM 2.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name RIM 1 (RIMS1 Antibody Abstract)
Pathways Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
Application Notes WB: not recommended
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000
IHC: 1 : 500
Comment

WB: Antibody 3 is recommended.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Immunocytochemistry (ICC) image for anti-Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 207-366) antibody (ABIN1742306) Indirect immunostaining on hippocampus neurons (dilution?1?:?500).
Immunohistochemistry (IHC) image for anti-Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 207-366) antibody (ABIN1742306) Indirect immunostaining of ribbon synapses in the outer plexiform layer of unfixed mo...
Product cited in: Limbach, Laue, Wang, Hu, Thiede, Hultqvist, Kilimann: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, 2011 (PubMed).