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  • 产品名称:anti-VAMP2antibody(Vesicle-AssociatedMembraneProtein2(Synaptobrevin2))(AA2-17)

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简单介绍:
anti-VAMP2antibody(Vesicle-AssociatedMembraneProtein2(Synaptobrevin2))(AA2-17)
详情介绍:
Immunogen Synthetic peptide SATAATVPPAAPAGEG (aa 2-17 in rat synaptobrevin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for VAMP 2
Cross-Reactivity (Details) no cross reactivity to VAMP 1 and VAMP 3
Purification antiserum
Alternative Name Synaptobrevin 2 (VAMP2 Antibody Abstract)
Background Synonyms: VAMP 2, p18-2
Research Area Tags/Labels
Pathways Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
Application Notes WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 1000
IHC: 1 : 500
Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 2-17) antibody (ABIN1742194) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 2-17) antibody (ABIN1742194) Immunocytochemistry on cultured hippocampus neurons (dilution 1 : 1000)
Immunohistochemistry (IHC) image for anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 2-17) antibody (ABIN1742194) Indirect immunolabeling of PFA fixed mouse hippocampus sections (dilution 1 : 500)
Product cited in: van Stegen, Dagar, Gottmann: "Release activity-dependent control of vesicle endocytosis by the synaptic adhesion molecule N-cadherin." in: Scientific reports, Vol. 7, pp. 40865, 2017 (PubMed).

Schaefer, Toral, Velez, Cox, Baker, Borcherding, Colgan, Bondada, Mashburn, Yu, Geddes, Tsang, Bassuk, Mahajan: "Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses." in: Investigative ophthalmology & visual science, Vol. 57, Issue 6, pp. 2509-21, 2016 (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, 2013 (PubMed).