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  • 产品名称:anti-Complexin4(CPLX4)antibody

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简单介绍:
anti-Complexin4(CPLX4)antibody
详情介绍:
Immunogen Recombinant full length protein of mouse complexin 4.
Clone 171H8
Isotype IgG1
Specificity Specific for complexin 4
Cross-Reactivity (Details) no cross reaction to other complexins.
Purification purified IgG
Alternative Name Complexin 4 (CPLX4 Antibody Abstract)
Background Synonyms: CPX 4
Application Notes WB: 1 : 1000 (ECL detection)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 2000
IHC-P: 1 : 200
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Complexin 4 (CPLX4) antibody (ABIN1742267) dilution: 1 : 1000 (ECL), sample: mouse retina extract
Immunohistochemistry (IHC) image for anti-Complexin 4 (CPLX4) antibody (ABIN1742267) Indirect immunostaining of mouse retina (dilution 1 : 2000). Complexin 4 positive str...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Complexin 4 (CPLX4) antibody (ABIN1742267) Indirect immunostaining of paraffin embedded mouse retina section with anti-complexin...
Product cited in: Vaithianathan, Henry, Akmentin, Matthews: "Functional roles of complexin in neurotransmitter release at ribbon synapses of mouse retinal bipolar neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 9, pp. 4065-70, 2015 (PubMed).

Landgraf, Mühlhans, Dedek, Reim, Brandst?tter, Ammermüller: "The absence of Complexin 3 and Complexin 4 differentially impacts the ON and OFF pathways in mouse retina." in: The European journal of neuroscience, Vol. 36, Issue 4, pp. 2470-81, 2012 (PubMed).

Xue, Stradomska, Chen, Brose, Zhang, Rosenmund, Reim: "Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 22, pp. 7875-80, 2008 (PubMed).