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  • 产品名称：anti-Syntaxin4(STX4)(AA1-273),(CytoplasmicDomain)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-Syntaxin4(STX4)(AA1-273),(CytoplasmicDomain)antibody
详情介绍：
Immunogen Recombinant cytoplasmic domain of rat syntaxin 4 (aa 1-273).
Clone 139-2
Isotype IgG1
Specificity Specific for syntaxin 4.
Purification purified IgG
Alternative Name Syntaxin 4 (STX4 Antibody Abstract)
Research Area Signaling
Pathways Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 100
IHC: not tested yet
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Syntaxin 4 (STX4) (AA 1-273), (Cytoplasmic Domain) antibody (ABIN1742220) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Syntaxin 4 (STX4) (AA 1-273), (Cytoplasmic Domain) antibody (ABIN1742220) Indirect immunostaining of HELA cells (dilution 1 : 100; red). Nuclei have been visua...
Product cited in: Milovanovic, Honigmann, Koike, G?ttfert, P?hler, Junius, Müllar, Diederichsen, Janshoff, Grubmüller, Risselada, Eggeling, Hell, van den Bogaart, Jahn: "Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains." in: Nature communications, Vol. 6, pp. 5984, 2015 (PubMed).