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  • 产品名称：anti-TenascinC(TNC)antibody

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简单介绍：
anti-TenascinC(TNC)antibody
详情介绍：
Immunogen Glycopeptidase F-treated tenascin from mouse brain. Epitope FNIII D domain
Clone 578
Isotype IgG
Specificity Specific for tenascin-C splice variants carrying the FNIII D domain.
Purification hybridoma supernatant
Alternative Name Tenascin-C (TNC Antibody Abstract)
Background Synonyms: TN-C, J1-200/220, Cytoactin
Pathways Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
Application Notes 1:10.000 recommended dilution
Comment

Tenascin-C variants detected by this antibody are downregulated during development and hardly detectable in ***** animals
Restrictions For Research Use only
Format Liquid
Reconstitution For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Buffer PBS
Handling Advice When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Tenascin C (TNC) antibody (ABIN1742432) dilution: 1 : 1000, sample: brain homogenate from new born rats
Immunocytochemistry (ICC) image for anti-Tenascin C (TNC) antibody (ABIN1742432) Indirect immunostaining of rat hippocampal neuron growing on astrocyte layer (dilutio...
Product cited in: Siddiqui, Horvat-Bröcker, Faissner: "The glia-derived extracellular matrix glycoprotein tenascin-C promotes embryonic and postnatal retina axon outgrowth via the alternatively spliced fibronectin type III domain TNfnD." in: Neuron glia biology, Vol. 4, Issue 4, pp. 271-83, 2010 (PubMed).

Rigato, Garwood, Calco, Heck, Faivre-Sarrailh, Faissner: "Tenascin-C promotes neurite outgrowth of embryonic hippocampal neurons through the alternatively spliced fibronectin type III BD domains via activation of the cell adhesion molecule F3/contactin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 15, pp. 6596-609, 2002 (PubMed).

Bartsch, Bartsch, Dörries, Faissner, Weller, Ekblom, Schachner: "Expression of tenascin in the developing and ***** cerebellar cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 12, Issue 3, pp. 736-49, 1992 (PubMed).

Stern, Norris, Bronner-Fraser, Carlson, Faissner, Keynes, Schachner: "J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo." in: Development (Cambridge, England), Vol. 107, Issue 2, pp. 309-19, 1990 (PubMed).

Gatchalian, Schachner, Sanes: "Fibroblasts that proliferate near denervated synaptic sites in skeletal muscle synthesize the adhesive molecules tenascin(J1), N-CAM, fibronectin, and a heparan sulfate proteoglycan." in: The Journal of cell biology, Vol. 108, Issue 5, pp. 1873-90, 1989 (PubMed).