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  • 产品名称:anti-Bassoon(BSN)(C-Term)antibody

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简单介绍:
anti-Bassoon(BSN)(C-Term)antibody
详情介绍:
Immunogen Recombinant protein containing the 330 C-terminal aa of rat bassoon.
Clone 219E1
Isotype IgG2b
Specificity Specific for bassoon.
Purification hybridoma supernatant
Alternative Name Bassoon (BSN Antibody Abstract)
Research Area Neurology
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet
Comment

ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20?°C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
Prolonged storage at 4?°C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Bassoon (BSN) (C-Term) antibody (ABIN1742310) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Bassoon (BSN) (C-Term) antibody (ABIN1742310) Immunocytochemistry on cultured hippocampus neurons (dilution 1 : 300). Immunoreactiv...
Product cited in: Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, 2016 (PubMed).

Hagmeyer, Mangus, Boeckers, Grabrucker: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." in: Neural plasticity, Vol. 2015, pp. 985083, 2015 (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kr?hnert, Sch?fer, Rammner, Koo, Cla?en, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, 2014 (PubMed).

Schr?der, Stellmacher, Romorini, Marini, Montenegro-Venegas, Altrock, Gundelfinger, Fejtova: "Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins." in: PLoS ONE, Vol. 8, Issue 3, pp. e58814, 2013 (PubMed).

Orenbuch, Shalev, Marra, Sinai, Lavy, Kahn, Burden, Staras, Gitler: "Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 12, pp. 3969-80, 2012 (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, 2012 (PubMed).

Morgans, Zhang, Jeffrey, Nelson, Burke, Duvoisin, Brown: "TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 45, pp. 19174-8, 2009 (PubMed).