anti-Amyloidbeta1-42(Abeta1-42)(AA37-42)antibody 抗体网

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产品名称：anti-Amyloidbeta1-42(Abeta1-42)(AA37-42)antibody
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产品厂商：SYSY
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简单介绍：

anti-Amyloidbeta1-42(Abeta1-42)(AA37-42)antibody

详情介绍：

Product details
Product details
Immunogen	Synthetic peptide corresponding to aa 37-42 of Abeta peptide 42 coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity	Specific for Abeta 42
Cross-Reactivity (Details)	weak cross-reactivity to Abeta 40 in westernblots that is not apparent in ELISA tests.
Purification	Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Target details
Target details
Alternative Name	Abeta 42 (Abeta 1-42 Antibody Abstract)
Background	Synonyms: Beta-App, ABPP

Application Details
Application Details
Application Notes	WB: 1 : 1000 (ECL detection) IP: not tested yet ICC: not tested yet IHC: 1 : 100 up to 1 : 500 IHC-P: 1 : 100 ELISA: yes, suitable only as capture antibody, ABIN1742439 is recommended detector antibody for sandwich-ELISA (protocol)
Comment	WB: Detects purified Abeta 42. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting.
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer	PBS
Handling Advice	Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage	-20 °C
Storage Comment	Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Images
Images
Supplier Images	ECL detection of different synthetic Abeta species with anti-Abeta 42 (dilution 1 : 1... anti-Amyloid beta 1-42 (Abeta 1-42) (AA 37-42) antibody (Image 2) Indirect immunostaining of an acetone fixed cryo-section from an Alzheimer′s disease ... Immunostaining of paraffin embedded brain section from an Alzheimer's patient (diluti... Images for product: anti-Amyloid beta 1-42 (Abeta 1-42) (AA 37-42) antibody ECL detection of different synthetic Abeta species with anti-Abeta 42 (dilution 1 : 1000) and a commercially available monoclonal anti-Abeta antibody (4G8, Covance, dilution: 1 : 3000). anti-Amyloid beta 1-42 (Abeta 1-42) (AA 37-42) antibody (Image 2) Indirect immunostaining of an acetone fixed cryo-section from an Alzheimer′s disease patient with anti Abeta 42 (dilution 1 : 100; red) and mouse anti Abeta-pE3 (cat. no. 218 011, dilution 1 : 200; green). Nuclei have been visualized by DAPI staining. Immunostaining of paraffin embedded brain section from an Alzheimer's patient (dilution 1 : 100). Immunoreactivity in amyloid plaques was revealed using diaminobenzidine as chromagen. Nuclei were counterstained with haematoxylin (blue).

References
References
Product cited in:	Hüttenrauch, Baches, Gerth, Bayer, Weggen, Wirths: "Neprilysin deficiency alters the neuropathological and behavioral phenotype in the 5XFAD mouse model of Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 44, Issue 4, pp. 1291-302, 2015 (PubMed). Guzmán, Bouter, Richard, Lannfelt, Ingelsson, Paetau, Verkkoniemi-Ahola, Wirths, Bayer: "Abundance of Aβ?-x like immunoreactivity in transgenic 5XFAD, APP/PS1KI and 3xTG mice, sporadic and familial Alzheimer's disease." in: Molecular neurodegeneration, Vol. 9, pp. 13, 2014 (PubMed). Reinert, Martens, Huettenrauch, Kolbow, Lannfelt, Ingelsson, Paetau, Verkkoniemi-Ahola, Bayer, Wirths: "Aβ38 in the brains of patients with sporadic and familial Alzheimer's disease and transgenic mouse models." in: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 4, pp. 871-81, 2014 (PubMed). Christensen, Huettenrauch, Mitkovski, Pradier, Wirths: "Axonal degeneration in an Alzheimer mouse model is PS1 gene dose dependent and linked to intraneuronal A? accumulation." in: Frontiers in aging neuroscience, Vol. 6, pp. 139, 2014 (PubMed). Bouter, Dietrich, Wittnam, Rezaei-Ghaleh, Pillot, Papot-Couturier, Lefebvre, Sprenger, Wirths, Zweckstetter, Bayer: "N-truncated amyloid β (Aβ) 4-42 forms stable aggregates and induces acute and long-lasting behavioral deficits." in: Acta neuropathologica, Vol. 126, Issue 2, pp. 189-205, 2013 (PubMed).