anti-MuscarinicAcetylcholineRec.2(AA207-388)antibody 抗体网

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产品名称：anti-MuscarinicAcetylcholineRec.2(AA207-388)antibody
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产品厂商：SYSY
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简单介绍：

anti-MuscarinicAcetylcholineRec.2(AA207-388)antibody

详情介绍：

Product details
Product details
Immunogen	Recombinant protein of human m2 receptor (aa 207-388).
Clone	2-1-155
Isotype	IgG
Specificity	Specific for muscarinic acetylcholine receptor 2.
Purification	purified IgG

Target details
Target details
Background	Synonyms: m-AChR-2

Application Details
Application Details
Application Notes	WB: 1 : 1000 (AP staining) IP: not tested yet ICC: not tested yet IHC: 1 : 100
Comment	This antibody detects also higher molecular weight bands, depending on the glycosilation state of the protein. The protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer	PBS
Handling Advice	Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage	-20 °C
Storage Comment	Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Images
Images
Supplier Images	anti-Muscarinic Acetylcholine Rec. 2 (AA 207-388) antibody anti-Muscarinic Acetylcholine Rec. 2 (AA 207-388) antibody (Image 2) A: Indirect immunolabeling of 3T3 cells (dilution 1 : 500). Nuclei were visualized by... dilution: 1 : 1000, sample: rat brain homogenate Images for product: anti-Muscarinic Acetylcholine Rec. 2 (AA 207-388) antibody anti-Muscarinic Acetylcholine Rec. 2 (AA 207-388) antibody anti-Muscarinic Acetylcholine Rec. 2 (AA 207-388) antibody (Image 2) A: Indirect immunolabeling of 3T3 cells (dilution 1 : 500). Nuclei were visualized by DAPI staining. B: Indirect immunolabeling of hippocampus neurons (dilution 1 : 500; red). Synaptic terminals were visualized with a primary labeled monoclonal synaptotagmin 1 antibody (cat. no. 105 311C5, dilution 1 : 300; green). dilution: 1 : 1000, sample: rat brain homogenate

References
References
Product cited in:	Duttaroy, Gomeza, Gan, Siddiqui, Basile, Harman, Smith, Felder, Levey, Wess: "Evaluation of muscarinic agonist-induced analgesia in muscarinic acetylcholine receptor knockout mice." in: Molecular pharmacology, Vol. 62, Issue 5, pp. 1084-93, 2002 (PubMed). Bernard, Laribi, Levey, Bloch: "Subcellular redistribution of m2 muscarinic acetylcholine receptors in striatal interneurons in vivo after acute cholinergic stimulation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 23, pp. 10207-18, 1998 (PubMed). Levey, Edmunds, Hersch, Wiley, Heilman: "Light and electron microscopic study of m2 muscarinic acetylcholine receptor in the basal forebrain of the rat." in: The Journal of comparative neurology, Vol. 351, Issue 3, pp. 339-56, 1995 (PubMed).