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  • 产品名称：anti-MAPTantibody(Microtubule-AssociatedProteintau)(AA3-214)

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  • 产品厂商：SYSY
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简单介绍：
anti-MAPTantibody(Microtubule-AssociatedProteintau)(AA3-214)
详情介绍：
Immunogen Recombinant mouse tau (aa 3-214).
Clone 248E5
Isotype IgG2a
Specificity Specific for tau.
Purification purified IgG. Azide was added before lyophilization.
Alternative Name tau (MAPT Antibody Abstract)
Pathways MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 200 up to 1 : 500
IHC: 1 : 100
Comment

WB: Detects the mouse protein with much greater sensitivity than the rat protein. The antibody binds phosphorylated and non-phosphorylated tau proteins.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein tau (MAPT) (AA 3-214) antibody (ABIN1742567) anti-Microtubule-Associated Protein tau (MAPT) (AA 3-214) antibody
Immunocytochemistry (ICC) image for anti-Microtubule-Associated Protein tau (MAPT) (AA 3-214) antibody (ABIN1742567) anti-Microtubule-Associated Protein tau (MAPT) (AA 3-214) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein tau (MAPT) (AA 3-214) antibody (ABIN1742567) anti-Microtubule-Associated Protein tau (MAPT) (AA 3-214) antibody (Image 3)
Product cited in: Gut, Beske, Hubbard, Lyman, Hamilton, McNutt: "Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury." in: PLoS ONE, Vol. 8, Issue 5, pp. e64423, 2013 (PubMed).

Ahmad, Wolber, Eckardt, Koch, Schmitt, Semechkin, Geis, Heckmann, Brüstle, McLaughlin, Sirén, Müller: "Functional neuronal cells generated by human parthenogenetic stem cells." in: PLoS ONE, Vol. 7, Issue 8, pp. e42800, 2012 (PubMed).