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  • 产品名称：anti-SynapsinII(SYN2)(AA440-458)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-SynapsinII(SYN2)(AA440-458)antibody
详情介绍：
Immunogen Synthetic peptide (aa 440-458 of mouse synapsin 2) coupled to KLH via an N-terminal added cysteine.
Clone 27E3
Isotype IgG1
Specificity Specific for synapsin 2
Purification purified IgG
Alternative Name Synapsin 2 (SYN2 Antibody Abstract)
Application Notes WB: 1 : 500 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet
Comment

WB: This antibody is less sensitive than the polyclonal rabbit antibody. Enriched synaptic vesicles are recommended for westernblotting.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS.
Buffer PBS, 0.02% sodium azide (= 0.02 μg)
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Synapsin II (SYN2) (AA 440-458) antibody (ABIN1742207) dilution: 1 : 1000, sample: synaptic vesicle fraction of rat brain (LP2)
Immunocytochemistry (ICC) image for anti-Synapsin II (SYN2) (AA 440-458) antibody (ABIN1742207) Indirect immunolabeling of cultured rat hippocampus neurons (dilution 1 : 500)
Immunohistochemistry (IHC) image for anti-Synapsin II (SYN2) (AA 440-458) antibody (ABIN1742207) Indirect immunostaining of PFA fixed mouse hippocampus section (dilution 1 : 200; red...
Product cited in: Acuna, Liu, Südhof: "How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, 2016 (PubMed).