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  • 产品名称:anti-Synaptogyrin1(SYNGR1)(AA170-234)antibody

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简单介绍:
anti-Synaptogyrin1(SYNGR1)(AA170-234)antibody
详情介绍:
Immunogen Recombinant rat synaptogyrin 1 (aa 170-234).
Clone 80-1
Isotype IgG1
Specificity Specific for synaptogyrin 1 with a weak side-reactivity towards tubulin.
Purification purified IgG
Alternative Name Synaptogyrin 1 (SYNGR1 Antibody Abstract)
Background Synonyms: p29
Research Area Neurology
Pathways Regulation of long-term Neuronal Synaptic Plasticity
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 100
Comment

IP: In immunoprecipitation (but not in other assays) tubulin coprecipitates with synaptogyrin 1 in about equal proportions. This is due to a direct binding of the antibody to tubulin. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742189 as detector antibody (protocol for sandwich-ELISA).

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Synaptogyrin 1 (SYNGR1) (AA 170-234) antibody (ABIN1742188) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Product cited in: von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, 2003 (PubMed).

Stenius, Janz, Südhof, Jahn: "Structure of synaptogyrin (p29) defines novel synaptic vesicle protein." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 2, pp. 1801-9, 1996 (PubMed).