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  • 产品名称:anti-ARPC5antibody(ActinRelatedProtein2/3Complex,Subunit5,16kDa)(AA19-24)

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简单介绍:
anti-ARPC5antibody(ActinRelatedProtein2/3Complex,Subunit5,16kDa)(AA19-24)
详情介绍:
Immunogen Recombinant full length p16-Arc. Epitope YDENKF (aa 19-24 in human p16-Arc).
Clone 323H3
Isotype IgG2a
Specificity Specific for p16-Arc.
Purification purified IgG. Azide was added before lyophilization.
Alternative Name p16-Arc (ARPC5 Antibody Abstract)
Background Synonyms: Arp C5
Pathways RTK Signaling, Regulation of Actin Filament Polymerization
Application Notes WB: 1 : 100 up to 1 : 2000 (AP staining)
IP: not tested yet
IHC: not tested yet
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Actin Related Protein 2/3 Complex, Subunit 5, 16kDa (ARPC5) (AA 19-24) antibody (ABIN1742556) anti-Actin Related Protein 2/3 Complex, Subunit 5, 16kDa (ARPC5) (AA 19-24) antibody
Product cited in: Oelkers, Vinzenz, Nemethova, Jacob, Lai, Block, Szczodrak, Kerkhoff, Backert, Schlüter, Stradal, Small, Koestler, Rottner: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, 2011 (PubMed).

Marion, Hoffmann, Holzer, Le Clainche, Martin, Sachse, Ganeva, Mangeat, Griffiths: "Ezrin promotes actin assembly at the phagosome membrane and regulates phago-lysosomal fusion." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 4, pp. 421-37, 2011 (PubMed).

Lladó, Timpson, Vilà de Muga, Moretó, Pol, Grewal, Daly, Enrich, Tebar: "Protein kinase Cdelta and calmodulin regulate epidermal growth factor receptor recycling from early endosomes through Arp2/3 complex and cortactin." in: Molecular biology of the cell, Vol. 19, Issue 1, pp. 17-29, 2008 (PubMed).

Steffen, Faix, Resch, Linkner, Wehland, Small, Rottner, Stradal: "Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes." in: Molecular biology of the cell, Vol. 17, Issue 6, pp. 2581-91, 2006 (PubMed).

Takamori, Holt, Stenius, Lemke, Gr?nborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gr?ter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, 2006 (PubMed).