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  • 产品名称：anti-alphaTubulinantibody(TUBA1)(AA443-449)

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简单介绍：
anti-alphaTubulinantibody(TUBA1)(AA443-449)
详情介绍：
Immunogen Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.
Clone 37B5
Isotype IgG1
Specificity Specific for alpha-tubulin.
Purification purified IgG. Azide was added before lyophilization.
Alternative Name alpha-Tubulin (TUBA1 Antibody Abstract)
Research Area Cytoskeleton
Pathways Microtubule Dynamics
Application Notes WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: 1 : 400
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) (AA 443-449) antibody (ABIN1742552) anti-alpha Tubulin (TUBA1) (AA 443-449) antibody
Immunocytochemistry (ICC) image for anti-alpha Tubulin (TUBA1) (AA 443-449) antibody (ABIN1742552) anti-alpha Tubulin (TUBA1) (AA 443-449) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) (AA 443-449) antibody (ABIN1742552) anti-alpha Tubulin (TUBA1) (AA 443-449) antibody (Image 3)
Product cited in: Luchkina, Huupponen, Clarke, Coleman, Kein?nen, Taira, Lauri: "Developmental switch in the kinase dependency of long-term potentiation depends on expression of GluA4 subunit-containing AMPA receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 11, pp. 4321-6, 2014 (PubMed).