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  • 产品名称：anti-Tyr-alpha-Tubulin(C-Term)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-Tyr-alpha-Tubulin(C-Term)antibody
详情介绍：
Immunogen Synthetic peptide GEEEGEEY (C-terminus of tyrosinated alpha-tubulin) coupled to key-hole limpet hemocyanin via glutaraldehyde.
Clone 20C6
Isotype IgG1
Specificity Specific for tyrosinated alpha-tubulin (tyr-tubulin).
Cross-Reactivity (Details) No cross reaction to glu-alpha-tubulin.
Purification purified IgG. Azide was added before lyophilization.
Application Notes WB: 1 : 100 up to 1 : 2000 (AP staining)
IHC: 1 : 200
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Tyr-alpha-Tubulin (C-Term) antibody (ABIN1742546) anti-Tyr-alpha-Tubulin (C-Term) antibody
Immunohistochemistry (IHC) image for anti-Tyr-alpha-Tubulin (C-Term) antibody (ABIN1742546) anti-Tyr-alpha-Tubulin (C-Term) antibody (Image 2)
Product cited in: Basheer, Brown, Ramesh, Begum, McCarley: "Sleep deprivation-induced protein changes in basal forebrain: implications for synaptic plasticity." in: Journal of neuroscience research, Vol. 82, Issue 5, pp. 650-8, 2005 (PubMed).

Wehland, Weber: "Turnover of the carboxy-terminal tyrosine of alpha-tubulin and means of reaching elevated levels of detyrosination in living cells." in: Journal of cell science, Vol. 88 ( Pt 2), pp. 185-203, 1988 (PubMed).