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  • 产品名称：anti-GRIA2antibody(GlutamateReceptor,Ionotropic,AMPA2)(AA278-292)

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简单介绍：
anti-GRIA2antibody(GlutamateReceptor,Ionotropic,AMPA2)(AA278-292)
详情介绍：
Immunogen Synthetic peptide (aa 278-292 in rat GluA 2, unprocessed precursor) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Clone 1404A10
Isotype IgG2b
Specificity Specific for GluA 2.
Purification purified IgG. Azide was added before lyophilization.
Alternative Name GluA 2 (GRIA2 Antibody Abstract)
Background Synonyms: AMPA 2, GluR 2
Pathways PI3K-Akt Signaling
Application Notes WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: not tested yet
Comment

IP:For most effective IP use the solubilization protocol described in this ELISA protocol. Consider that protein-protein interaction may be affected.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) (AA 278-292) antibody (ABIN1742378) dilution: 1 : 1000; sample: rat hippocampus homogenate
Product cited in: Jedlicka, Vnencak, Krueger, Jungenitz, Brose, Schwarzacher: "Neuroligin-1 regulates excitatory synaptic transmission, LTP and EPSP-spike coupling in the dentate gyrus in vivo." in: Brain structure & function, Vol. 220, Issue 1, pp. 47-58, 2015 (PubMed).