anti-alphaTubulinantibody(TUBA1)(AA419-435) 抗体网

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产品名称：anti-alphaTubulinantibody(TUBA1)(AA419-435)
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anti-alphaTubulinantibody(TUBA1)(AA419-435)

详情介绍：

Product Details anti-alpha Tubulin Antibody
Product Details anti-alpha Tubulin Antibody
Immunogen	Synthetic peptide SEAREDMAALEKDYEEV (aa 419-435 in human alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.
Clone	3A2
Isotype	IgG1
Specificity	Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).
Purification	purified IgG. Azide was added before lyophilization.

Target Details alpha Tubulin
Target Details alpha Tubulin
Alternative Name	alpha-Tubulin (TUBA1 Antibody Abstract)
Research Area	Cytoskeleton
Pathways	Microtubule Dynamics

Application Details
Application Details
Application Notes	WB: 1 : 1000 up to 1 : 50000 (AP staining) ICC: 1 : 500 up to 1 : 1000 IHC-P: 1 : 200
Comment	ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer	PBS, 0.02% sodium azide
Preservative	Sodium azide
Precaution of Use	This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice	Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage	-20 °C
Storage Comment	Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Images
Images
Supplier Images	anti-alpha Tubulin (TUBA1) (AA 419-435) antibody Indirect immunostaining of PFA-fixed mouse brain section (dilution 1 : 500; green). N... Indirect immunostaining of PFA-fixed paraffin embedded mouse spleen section (dilution... dilution: 1 : 1000, sample: rat brain homogenate Images for product: anti-alpha Tubulin (TUBA1) (AA 419-435) antibody anti-alpha Tubulin (TUBA1) (AA 419-435) antibody Indirect immunostaining of PFA-fixed mouse brain section (dilution 1 : 500; green). Nuclei have been visualized by DAPI staining (blue). Indirect immunostaining of PFA-fixed paraffin embedded mouse spleen section (dilution 1 : 500; green). Nuclei have been visualized by DAPI staining (blue). dilution: 1 : 1000, sample: rat brain homogenate

References for anti-alpha Tubulin antibody (ABIN1742547)
References for anti-alpha Tubulin antibody (ABIN1742547)
Product cited in:	Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, 2015 (PubMed). Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, 2014 (PubMed). Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, 2013 (PubMed).