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  • 产品名称：anti-gamma-aminobutyricAcid(GABA)AReceptor,alpha1(GABRA1)(AA28-43)antibody

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简单介绍：
anti-gamma-aminobutyricAcid(GABA)AReceptor,alpha1(GABRA1)(AA28-43)antibody
详情介绍：
Immunogen Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Clone 53D3
Isotype IgM
Specificity Specific for GABA-A receptor alpha1.
Purification purified IgM. Azide was added before lyophilization.
Alternative Name GABA-A Receptor Alpha1 (GABRA1 Antibody Abstract)
Background Synonyms: GABA(A) R alpha1
Application Notes WB: 1 : 1000 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500
Comment

ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) antibody (ABIN1742455) dilution: 1 : 1000, sample: unboiled synaptic membrane fraction of rat brain (LP1)
Immunocytochemistry (ICC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) antibody (ABIN1742455) Indirect immunostaining of hippocampus neurons with anti-GABA-A receptor α1 (dilution...
Immunohistochemistry (IHC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) antibody (ABIN1742455) Indirect immunostaining of mouse cerebellum and main olfactory bulb vibratome section...
Product cited in: Acuna, Liu, Südhof: "How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, 2016 (PubMed).