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  • 产品名称：anti-Glu-alpha-Tubulin(C-Term)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-Glu-alpha-Tubulin(C-Term)antibody
详情介绍：
Immunogen Synthetic peptide VDSVEGEGEEEGEE (14 C-terminal aa of detyrosinated alpha-tubulin) coupled to BSA via glutaraldehyde. Epitope X-EE including the terminal alpha-carboxylate group. All linkages in the peptide involve alpha-peptide bonds.
Clone 1D5
Isotype IgG1
Specificity Specific for detyrosinated alpha-tubulin (glu-tubulin) and polyglutamylated tubulin (also beta-tubulin).
Cross-Reactivity (Details) No cross reaction to tyrosinated tubulin.
Purification purified IgG. Azide was added before lyophilization.
Application Notes WB: 1 : 100 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200
IHC: 1 : 200
Comment

WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Glu-alpha-Tubulin (C-Term) antibody (ABIN1742545) anti-Glu-alpha-Tubulin (C-Term) antibody
Immunohistochemistry (IHC) image for anti-Glu-alpha-Tubulin (C-Term) antibody (ABIN1742545) anti-Glu-alpha-Tubulin (C-Term) antibody (Image 2)
Product cited in: Zink, Grosse, Freikamp, B?nfer, Müksch, Jacob: "Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells." in: Journal of cell science, Vol. 125, Issue Pt 24, pp. 5998-6008, 2013 (PubMed).

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Zhang, Gilquin, Khochbin, Matthias: "Two catalytic domains are required for protein deacetylation." in: The Journal of biological chemistry, Vol. 281, Issue 5, pp. 2401-4, 2006 (PubMed).

Rosales-Nieves, Johndrow, Keller, Magie, Pinto-Santini, Parkhurst: "Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino." in: Nature cell biology, Vol. 8, Issue 4, pp. 367-76, 2006 (PubMed).

Yoon, Shin, Mercurio: "Hypoxia stimulates carcinoma invasion by stabilizing microtubules and promoting the Rab11 trafficking of the alpha6beta4 integrin." in: Cancer research, Vol. 65, Issue 7, pp. 2761-9, 2005 (PubMed).

Van der Heiden, Groenendijk, Hierck, Hogers, Koerten, Mommaas, Gittenberger-de Groot, Poelmann: "Monocilia on chicken embryonic endocardium in low shear stress areas." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 1, pp. 19-28, 2005 (PubMed).

Rüdiger, Rüdiger, Wehland, Weber: "Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin." in: European journal of cell biology, Vol. 78, Issue 1, pp. 15-20, 1999 (PubMed).

Wheatley, Feilen, Yin, Wheatley: "Primary cilia in cultured mammalian cells: detection with an antibody against detyrosinated alpha-tubulin (ID5) and by electron microscopy." in: Journal of submicroscopic cytology and pathology, Vol. 26, Issue 1, pp. 91-102, 1994 (PubMed).