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  • 产品名称：Anti-PolyvalentHRPDABDetectionSystem

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  • 产品厂商：SpringBio
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简单介绍：
Anti-PolyvalentHRPDABDetectionSystem
详情介绍：
Characteristics The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated rabbit or mouse primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Protocol 1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Add enough drops of Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
3. Perform appropriate pretreatment if required. Wash 3 times in buffer.
4. Apply Protein Block (if required) and incubate for 10 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
5. Apply primary antibody and incubate according to manufacturer’s protocol.
6. Wash 4 times in buffer. Apply Biotinylated Goat Anti-Polyvalent and incubate for 10 minutes at room temperature. Wash 4 times in buffer.
7. Apply Streptavidin Peroxidase and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Add 30 µl (1 drop) DAB Chromogen to 1.5 ml (50 drops) of DAB Substrate, mix by swirling and apply to tissue. Incubate for 1-10 minutes. Rinse 4 times in buffer.
9. Apply counterstain according to manufacturer’s instructions. 10. Dehydrate if required and coverslip.
Restrictions For Research Use only
Format Liquid
Precaution of Use 1. Take reasonable precautions when handling reagents. Wear appropriate Personal Protective Equipment.
2. Avoid contact of reagents with eyes, mucous membranes and skin. If reagents come into contact with sensitive areas, wash with copious amounts of water.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The user must validate any other storage conditions. The reagents must be returned to the storage conditions identified above immediately after use. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.