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Characteristics
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6-CFDA has been used to differentiate viable cells from apoptotic cells. • Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enters cells and is hydrolyzed more slowly than FDA. CFDA and FDA hydrolysis obey Michaelis-Menten kinetics with Km values of around 19 and 2 µM, respectively.
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