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Characteristics
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KEY FEATURES: SPDP contains a dithiopyridyl group which is reactive towards cysteines (-SH groups) and an NHS group, which is useful when targeting primary amines (-NH2 groups) SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed Contains a disulfide linkage in its spacer arm with a length of 6.8 A° The spacer is cleavable with reducing agent, such as DTT, TCEP, etc.APPLICATIONS: Reversible crosslinking of proteins N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains Useful for adding sulfhydryl groups to proteins by attachment to lysine residues Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.
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