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Characteristics
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Biotin-LC-LC-NHS is a useful reagent for biotinylation of primary amines under mild conditions (pHca. 6.5-8.5). Some examples of primary amines for biotinylation include lysines on the surface of proteins, antibodies, etc. Inserts a 14-atom spacer between biotin and the target ligand, thus minimizing the steric hindrance in binding avidin or streptavidin to some biotinylated compounds and increase sensitivity for detection.
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