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  • 产品名称：ActiveHumanCaspasesSetI

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  • 产品厂商：Biovision
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简单介绍：
ActiveHumanCaspasesSetI
详情介绍：
Unit Definition One unit of the recombinant human caspase-3, -6, -7, and -8 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA, VEID-pNA, DEVD-pNA, and IETD-pNA, respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Sub Type Cocktail
Background Caspases are known to play an important role in apoptosis. Caspases exist in cells as inactive pro-enzymes and mature by proteolysis to yield large and small subunits. Active caspases are heterotetramers consisting of two large and two small subunits.
Synonyms: Active Human Caspases Set, active human caspase, active caspases, human caspases, caspase enzyme, caspase protein,
Application Notes Highly active human caspases were expressed in E. coli and purified. Active caspases are useful for screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, and as a positive control in caspase activity assays and Western blot analysis. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase activity assay protocol, please refer to Caspase Activity Assay Kits.
Comment

physical form description: Lyophilized powder
Contains active caspase-3/-6/-7/-8.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 0.1-1 unit per μl in PBS + 15 % glycerol
Buffer 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1 % Chaps, 10 mM EDTA, 5 % Glycerol, and 10 mM DTT.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Expiry Date 12 months