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  • 产品名称：CaspasesGroupIII,ActiveHuman

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  • 产品厂商：Biovision
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CaspasesGroupIII,ActiveHuman
详情介绍：
Unit Definition One unit of the active recombinant caspase-6,-8,-9 & -10 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (for caspase-6), IETD-pNA (for caspase-8 & -10), and LEHD-pNA (for caspase-9), respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Sub Type Cocktail
Background Caspase family consists of more than 10 members. Based on their extended substrate specificities, these enzymes were divided into three major groups: Group I (contains caspase-1,-4,-5), Group II (contains caspase-2,-3,-7), and Group III (contains caspase-6,-8,-9,-10). Evidences have suggested that Group I caspases are involved primarily in the production of inflammatory cytokines, while group II and III enzymes function in apoptosis as effectors and upstream activators, respectively. The Group III recombinant caspases were expressed in E. coli and routinely tested for their ability to enzymatically cleave the substrate VEID-pNA (for caspase-6), IETD-pNA (for caspase-8 & -10) and LEHD-pNA (for caspase-9), respectively.
Synonyms: Active Human Caspases group, active human caspase, active caspases, human caspases, caspase enzyme, caspase protein,
Application Notes Active recombinant caspases are useful for screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, and as positive controls in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase activity assay protocol, please refer to Fluorometric or Colorimetric Caspase Assay Kits.
Comment

physical form description: Lyophilized powder
Group III contains active caspase-6/-8/-9/-10.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 1 unit per μl in PBS containing 15 % glycerol
Buffer 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1 % Chaps, 10 mM EDTA, 5 % Glycerol, and 10 mM DTT.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Expiry Date 12 months
Product cited in: Cheng, Chen, Best, Bloom, Pintel, Qiu: "The capsid proteins of Aleutian mink disease virus activate caspases and are specifically cleaved during infection." in: Journal of virology, Vol. 84, Issue 6, pp. 2687-96, 2010 (PubMed).