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  • 产品名称：ProteinGSepharoseColumn

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  • 产品厂商：Biovision
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简单介绍：
ProteinGSepharoseColumn
详情介绍：
Purpose Protein G Sepharose Column for affinity purification of antibodies.
Specificity High binding capacity = Binding of IgG >20 mg human or rabbit IgG/ml Protein G-Sepharose.
Minimal Leaching of the Ligand
Flow Rate Tested* = 2.07 ml/min.
*Test condition: = Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
Usage = Reusable for up to 10 times without significant loss of binding capacity.
Characteristics Protein G Sepharose is prepared by covalently coupling Recombinant Protein G (contains three IgG binding domain) to 6 % crosslinkedsepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding could be greater than 10 mg of human IgG per mL of wet bead.
ProductDetails: Bead Ligand Protein G
ProductDetails: Bead Matrix Sepharose beads
ProductDetails: Bead Size 90 µm
Application Notes - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Restrictions For Research Use only
Format Liquid
Buffer Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in PBS with 0.02% sodium azide. 
Storage 4 °C
Expiry Date 12 months
Product cited in: Traini, Piccolo, Tinari, Rossi, La Sorda, Spinella, Bagnato, Lattanzio, DEgidio, Di Risio, Tomao, Grassadonia, Piantelli, Natoli, Iacobelli: "Inhibition of tumor growth and angiogenesis by SP-2, an anti-lectin, galactoside-binding soluble 3 binding protein (LGALS3BP) antibody." in: Molecular cancer therapeutics, Vol. 13, Issue 4, pp. 916-25, 2014 (PubMed).