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  • 产品名称：Mouseanti-HumanIg(Chainkappa),(LightChain)Antibody

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  • 产品厂商：SpringBio
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简单介绍：
Mouseanti-HumanIg(Chainkappa),(LightChain)Antibody
详情介绍：
Immunogen B-lymphoma cells
Clone SPM508
Isotype IgG1
Characteristics Cellular Localization: Cytoplasm
Purification Purified by Protein A/G
Alternative Name Immunoglobulin Ig
Background Immunoglobulins belong to a group of related glyco proteins which make up 20 % of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit, while, IgM is a pentamer. IgA may also form polymers. Kappa light chain contains one immunoglobulin-like domain.
Molecular Weight 25 kDa
Gene ID 3514
Application Notes IHC Dilution: 1:200
Comment

Positive Control:
IHC (p): Tonsil
WB: Spleen cell lysate
Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only
Format Liquid
Buffer mouse monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C
Supplier Images
Immunohistochemistry (IHC) image for Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1688919) Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody
Immunohistochemistry (IHC) image for Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1688919) Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 2)
Immunohistochemistry (IHC) image for Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1688919) Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 3)
Immunohistochemistry (IHC) image for Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1688919) Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 4)
Western Blotting (WB) image for Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1688919) Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 5)
Immunohistochemistry (IHC) image for Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1688919) Mouse anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 6)