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  • 产品名称：ProteinGAgaroseBeads/Resin

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  • 产品厂商：SinoBiological
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简单介绍：
ProteinGAgaroseBeads/Resin
详情介绍：
Purpose Antibody Purification Reagent
Characteristics Ligand: Recombinant Streptococcal Protein G
Dynamic binding capacity: ~20 mg/ml
Matrix: Spherical, 4% agarose
Average particle size: 90um (45-165um)
Maximum linear flow rate: >150 cm/h
Maximum Pressure: 0.3 MPa
pH stability
Long term: pH 3-9
Short term: pH 2-10
ProductDetails: Bead Ligand Protein G
ProductDetails: Bead Matrix Agarose beads
ProductDetails: Bead Size 90 µm
Application Notes Column: 5x 50 mm, bed volume 1.0 ml
Sanitize the packed column with 2% Hibitane/20% ethanol or with 70% ethanol
Comment

Protein G Agarose Beads / Resin was produced by immobilizing recombinant protein G to 4% cross-linked agarose matrix. It is designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins or recombinant proteins containing Fc regions from biological fluids or cell culture media.
Protocol The column was equilibrated with biding buffer, then apply the sample at fow rate of 0.3 ml/min, when the flowthrough concentration reach 0.19 mg/min stop loading and wash the columm with biding buffer, when asorbency of 280 nm decrease to base line, elute with elution buffer.
Restrictions For Research Use only
Format Liquid
Buffer Binding Buffer: 50 mM Tris, 100 mM NaCl, pH 8.0
Elution Buffer: 100 mM glycin, 10 mM NaCl, pH 3.0
Handling Advice Do not freeze the resin.
Storage 4 °C
Storage Comment For storage, keep the medium at 2°C to 8°C in a suitable bacteriostat, e.g. 20% ethanol and/or 0.02% sodium azide.