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Purpose
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Kit for manufacturing of immuno affinity columns by immobilization of ligands with primary and secondary amines using activated cellulose beads
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Characteristics
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Kit for manufacturing of immuno affinity columns by immobilization of ligands with primary and secondary amines using activated cellulose beads, The BioTeZ-activated cellulose beads provide an easy way to immobilize ligands. The coupling reaction is simple to carry out. Many applications are possible, e.g. immobilization of proteins, peptides and nucleic acids. Precondition for coupling is that ligands contain primary and secondary amines. BioTeZ-activated cellulose beads enables customized immuno affinity gels and col-umns with the following properties: ? reusable, ? excellent flow behaviour without extra pressure, ? little abrasion. The coupled product has a high chemical stability to commonly used aqueous solu-tions. Usable with non-ionic detergents, denaturing solvents, e.g. 6 M guanidine hydrochloride and 8 M urea. Stable in organic solvents, such as 50?% dioxane and 50?% dimethylformamide. To autoclave is not recommended.
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Components
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Pre-activated cellulose beads, Coupling solution, Block solution, Wasch buffer 1 and 2, Storage buffer, empty columns
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Material not included
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Glass frit (G1 or G2), filter flask, pump/water-jet pump, pipettes or pipetting equipment, rotating mixer (recommended) , distilled or deionised water
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