产品详情
查看大图

  • 产品名称：HeLaCellLysate(H2O2stimulated)

  • 产品型号：
  • 产品厂商：BioLeaf
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
HeLaCellLysate(H2O2stimulated)
详情介绍：
Characteristics HeLa Cell Lysate (H2O2 stimulated) for use in the research laboratory
Components A 100 ul vial ofunstimulated cellsis supplied in addition to the 100 ul vial of stimulated cells.
ProductDetails: Lysate Fraction Whole Cell Lysate
Lysate Treatment 30 minutes in the presence of 1mM H2O2
Lysate Type Cell Lysate
Lysed Cells HeLa Cells
Application Notes Boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for Immunoblot analysis.
Restrictions For Research Use only
Buffer Cells were cultured for 30 minutes in the presence of 1 mM H2O2 (simulated cells only) and lysed in Lysis buffer (20 mM HEPES, pH 7.2, 20 mM Phosphoglycerate, 150 mM NaCl, 10 % glycerol, 1 % NP-40, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 5 mM NaF, 1 mM PMSF, 5 μg/mL Pepstatin A, 5 μg/mL Aprotinin, 5 μg/mL Leupeptin). They were then diluted with reducing sample buffer (60 mM Tris, pH 6.8, 6 % glycerol, 2 % SDS, 0.2 % bromphenolblue, 200 mM 2-mercaptoethanol).