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Comment
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Protease Inhibitor Cocktail is a mixture of 6 different protease inhibitors, this ensures inhibition of a broad range of proteases and esterases, including aspartase, cysteine, serine and aminopeptidase. It is liquid in 1ml of DMSO. Phosphatase Inhibitor Cocktail is a special mixture of 5 different phosphatase inhibitors, it has a good inhibitory effect on protein tyrosine phosphatase, acid and alkaline phosphatase. PMSF (100 mM) is used for inhibiting serine kind protease irreversibly, eg: trypsase, chymotrypsin, callicrein, subtilisin and thrombin. It also can be used for inhibiting homocysteine protease-papain.
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Sample Preparation
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Dilute the three components at 1:100 in an appropriate lysis buffer and mix thoroughly, then extract the sample protein, or according to the purpose of experiment, and the cultured cells and tissues, grope and optimize the experimental conditions through experiment.
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