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  • 产品名称：TissueMitochondriaIsolationKit

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  • 产品厂商：Biospes
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简单介绍：
TissueMitochondriaIsolationKit
详情介绍：
Background 1. Tissue Mitochondria Isolation Kit is designed for quick and convenient isolating mitochondria of animal tissues. 2. While isolating the mitochondria, this kit obtains the mitochondria excluded cytoplasmic protein, which can be used for researching the release of mitochondria proteins (e.g. cytochrome c, etc.) to cytoplasm. 3. The purity of the obtained mitochondria is high, and the majority has intact inner and outer membrane with physiological function. 4. After lysing with Mitochondria Lysis Buffer, the mitochondria can be used for SDS-PAGE, Western blot, Dimensional electrophoresis and Immunoprecipitation. 5. This kit is enough for 50-100 assays if the weight of per tissue is 50-100 mg. Kit Components Components
Plate Strips (12 x 8)
Sample Preparation
  1. Prepare solutions: thaw three reagents of the kit, put them on ice once completely dissolved, and mix thoroughly. If want to obtain the mitochondria protein, according to the number of sample, take the proper volume of MIR A for use, several minutes before adding to sample, add properly diluted Enzyme Inhibitor (
Restrictions For Research Use only
Preservative Sodium azide, Thimerosal (Merthiolate)
Storage -20 °C
Storage Comment Mitochondria Isolation Reagent A (MIR A) 60 ml Store at -20 °C for one year. Mitochondria Lysis Buffer B (MLB B) 20 ml Store at -20 °C for one year. Enzyme Inhibitor 1 ml Store at -20 °C for one year.
Expiry Date 12 months