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Purpose
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A quantitative assay for in vitro the determination of mouse NGAL in urine, plasma, serum, tissue extracts or culture media.
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Sample Type
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Urine, Plasma, Serum, Tissue Culture Supernatant
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Analytical Method
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Quantitative
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Detection Method
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Colorimetric
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Specificity
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With the Mouse NGAL ELISA Kit, urine, plasma or serum samples can be analysed in a standard laboratory with conventional ELISA equipment. All components are stored at 4ºC and all incubations are performed at room temperature. The kit contains ready-to-use reagents and a pre-coated 96-well ELISA plate, which greatly reduces the hands-on time. The simple test protocol permits high reproducibility with a total assay time under 4 hours.
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Sensitivity
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0.75 pg/mL
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Components
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1: Microwell plate coated with anti-NGAL (mouse) antibody (96 wells)
2: 5X Sample Diluent
3: 2 containers of lyophilized Mouse NGAL calibrators.
4: 25X Wash Solution Concentrate
5: Biotinylated Mouse NGAL Antibody
6: HRP-Streptavidin
7: TMB Substrate
8: Stop Solution
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