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  • 产品名称：HIV-1NefProtein

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  • 产品厂商：BioAcademia
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简单介绍：
HIV-1NefProtein
详情介绍：
Purity > 90 % by SDS-PAGE (CBB staining)
Background HIV-1 Nef is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears the most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cells and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in endocytosis and degradation of the cell surface receptor proteins such as CD4 and MH4 which are important for AIDS virus infection. HIV-1 Nef protein was over-expressed as a recombinant protein in E. coli with a plasmid carrying the nef gene of HIV-1 virus, subtype B and highly purified by several steps of chromatography. Its molecular size is 27kD, same as that of Nef purified from AIDS virus particles.
Application Notes 1) For functional studies of HIV-1 Nef protein
2) Used as standard for the titration analysis of HIV-1 Nef antigen
3) It can be used as Nef antigen in detection of anti-HIV-1 Nef antibody by Western blotting or ELISA.
Restrictions For Research Use only
Format Liquid
Concentration 0.48 mg/mL
Buffer 50 % glycerol, 20 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for HIV-1 Nef protein (ABIN2452196) HIV-1 Nef protein
Product cited in: Arora, Fredericksen, Garcia: "Nef: agent of cell subversion." in: Microbes and infection / Institut Pasteur, Vol. 4, Issue 2, pp. 189-99, 2002 (PubMed).

Fackler, Baur: "Live and let die: Nef functions beyond HIV replication." in: Immunity, Vol. 16, Issue 4, pp. 493-7, 2002 (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, 1986 (PubMed).