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  • 产品名称：HIV-1GagP17Protein

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  • 产品厂商：BioAcademia
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简单介绍：
HIV-1GagP17Protein
详情介绍：
Purity > 90 % by SDS-PAGE (CBB staining)
Background HIV-1 Gag p17 is the matrix protein of AIDS virus, HIV-1, and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable for reproduction of AIDS virus and is essential component of AIDS virus particles. This protein was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p17 coding region of HIV-1 virus, subtype B, and was highly purified by several steps of chromatography. Its molecular weight is 17 kD, same as that of p17 purified from AIDS virus particles.
Application Notes 1) It can be used as p17 antigen in detection of anti-HIV-1 p17 antibody in Western blotting or ELISA.
2) It can be used as a standard for quantitative analysis of HIV-1 p17 antigen.
3) It can be used in studies of structure and function of AIDS virus as a matrix protein that constitutes HIV-1 core.
Restrictions For Research Use only
Format Liquid
Concentration 0.23 mg/mL
Buffer 50 % glycerol, 20 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for HIV-1 Gag P17 protein (ABIN2452193) HIV-1 Gag P17 protein
Product cited in: Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, 1998 (PubMed).

Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, 1996 (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, 1986 (PubMed).