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  • 产品名称：HIV-1GagP15Protein

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  • 产品厂商：BioAcademia
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简单介绍：
HIV-1GagP15Protein
详情介绍：
Purity > 90 % by SDS-PAGE (CBB staining)
Background HIV-1 Gag p15 is processed by digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA. HIV-1 Gag p15 was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus, subtype B, and highly purified by several steps of chromatography. Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles.
Application Notes 1) It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.
2) It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.
3) It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.
4) It can be used as a standard for the quantitative analysis of HIV-1 p15 antigen.
Restrictions For Research Use only
Format Liquid
Concentration 0.42 mg/mL
Buffer 50 % glycerol, 20 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for HIV-1 Gag P15 protein (ABIN2452192) HIV-1 Gag P15 protein
Product cited in: Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, 1998 (PubMed).

Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, 1996 (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, 1986 (PubMed).