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  • 产品名称：T4SSB(Active)Protein

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  • 产品厂商：BioAcademia
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简单介绍：
T4SSB(Active)Protein
详情介绍：
Characteristics The absence of endonucleases and exonucleases was confirmed.
Purity > 95 % of protein determined by SDS-PAGE (CBB staining)
Background T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.
UniProt P03695
Application Notes 1. Promoting DNA replication and recombination by stabilizing single-stranded DNA
2. Increase specificity and yields of long PCR products
Restrictions For Research Use only
Format Liquid
Concentration 10 mg/mL
Buffer 20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.5 mM dithiothreitol, 1 mM EDTA, 50 % glycerol
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Schwarz, Hansen-Hagge, Bartram: "Improved yields of long PCR products using gene 32 protein." in: Nucleic acids research, Vol. 18, Issue 4, pp. 1079, 1990 (PubMed).

Product cited in: Bittner, Burke, Alberts: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, 1979 (PubMed).

Alberts, Frey: "T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA." in: Nature, Vol. 227, Issue 5265, pp. 1313-8, 1970 (PubMed).