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  • 产品名称：SSB(Active)Protein

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  • 产品厂商：BioAcademia
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简单介绍：
SSB(Active)Protein
详情介绍：
Purity > 95 % purity as determined by SDS-PAGE (CBB staining)
Background E.coli single-stranded DNA binding protein (SSB) binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination in vivo. The SSB gene was expressed as the recombinant protein in E.coli highly purified. The molecular mass is 18.9 kDa.
UniProt P0AGE0
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes 1. Functional single-stranded DNA-binding protein for studying DNA replication and recombination
2. Enhancement of the specificity and yield of PCR
Comment

The absence of endonucleases and exonucleases was confirmed.
Restrictions For Research Use only
Format Liquid
Concentration 5 mg/mL
Buffer 20 mM Tris-HCl ( pH 7.6), 200 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50 % glycerol
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Krauss, Sindermann, Schomburg, Maass: "Escherichia coli single-strand deoxyribonucleic acid binding protein: stability, specificity, and kinetics of complexes with oligonucleotides and deoxyribonucleic acid." in: Biochemistry, Vol. 20, Issue 18, pp. 5346-52, 1982 (PubMed).

Weiner, Bertsch, Kornberg: "The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication." in: The Journal of biological chemistry, Vol. 250, Issue 6, pp. 1972-80, 1975 (PubMed).