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  • 产品名称：AdenylateCyclase(ADCY)(Active)Protein

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  • 产品厂商：BioAcademia
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简单介绍：
AdenylateCyclase(ADCY)(Active)Protein
详情介绍：
Purity > 90 % purity by SDS-PAGE (CBB staining)
Background Adenylate cyclase is a toxin produced by a gram-negative coccobacillus, Bordetella pertusis. This toxin penetrates into the animal cells and produces cAMP from ATP in the cytoplasm. It belongs to the RTX family of toxins produced by many gram-negative bacteria. The molecular weight is 177 kDa This product is a recombinant toxin of Bordetella pertussis Tohama strain expressed in E. coli from the structural gene cyaA and its activator gene cyaC and purified by the method of Friedman.
UniProt P0DKX7
Application Notes Functionale studies on the cAMP- signaling pathway (10~100 ng/mL)
2) Antigen for western blotting and ELISA
3) SDS-PAGE marker as native adenylate cyclase Activity tested: Addition of the 10~100 ng/mL adenylate cyclase toxin to medium increased significant levels of cellular cAMP levels in CHO and human monocyte cells.
Restrictions For Research Use only
Format Liquid
Concentration 550 µg/mL
Buffer 50 mM Tris-HCl ( pH 8.0), 6 M urea, 150 mM NaCl
Storage -80 °C
Supplier Images
SDS-PAGE (SDS) image for Adenylate Cyclase (ADCY) (Active) protein (ABIN2452164) Adenylate Cyclase (ADCY) (Active) protein
Product cited in: Westrop, Hormozi, Da Costa, Parton, Coote: "Bordetella pertussis adenylate cyclase toxin: proCyaA and CyaC proteins synthesised separately in Escherichia coli produce active toxin in vitro." in: Gene, Vol. 180, Issue 1-2, pp. 91-9, 1997 (PubMed).

Friedman: "Bordetella pertussis adenylate cyclase: isolation and purification by calmodulin-sepharose 4B chromatography." in: Infection and immunity, Vol. 55, Issue 1, pp. 129-34, 1987 (PubMed).