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  • 产品名称：BotulinumC3Enzyme(Active)Protein

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  • 产品厂商：BioAcademia
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简单介绍：
BotulinumC3Enzyme(Active)Protein
详情介绍：
Purity > 90 % purity by SDS-PAGE (CBB staining)
Background This product was highly purified from the supernatant of the culture of Clostridium botulinum type C by cation exchange, hydroxyapatite and gel filtration columns. This toxin ADP-ribosylates Rho and Rac, which are low-molecular weight GTP-binding proteins of animal cells, and interrupts the downstream signal transduction pathways. The molecular weight is 24 kDa.
UniProt P15879
Application Notes 1) For the studies on the Rho-dependent signaling pathways in animal cells in vivo and in vitro.
2) ADP-ribosylation of small GTP proteins, such as Rho and Rac proteins.
Activity: Stimulation of human keratinocytes in culture was observed at 50 ng/mL.
Comment

No toxic effect was observed in two mice injected with 5 ug of C3 enzyme, showing little contamination of lethal neurotoxins from the botulinus culuture in this preparation.
Restrictions For Research Use only
Format Liquid
Concentration 1.33 mg/mL
Buffer 5 mM sodium phosphate buffer ( pH 6.0), 50 % glycerol
Storage -20 °C/-80 °C
Storage Comment -20 C (For long term storage: -70 C)
Supplier Images
SDS-PAGE (SDS) image for Botulinum C3 Enzyme (Active) protein (ABIN2452165) Botulinum C3 Enzyme (Active) protein
Product cited in: Fiorentini, Gauthier, Donelli, Boquet: "Bacterial toxins and the Rho GTP-binding protein: what microbes teach us about cell regulation." in: Cell death and differentiation, Vol. 5, Issue 9, pp. 720-8, 1999 (PubMed).

Moriishi, Syuto, Yokosawa, Oguma, Saito: "Purification and characterization of ADP-ribosyltransferases (exoenzyme C3) of Clostridium botulinum type C and D strains." in: Journal of bacteriology, Vol. 173, Issue 19, pp. 6025-9, 1991 (PubMed).