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  • 产品名称：anti-NUF2antibody(NUF2,NDC80KinetochoreComplexComponent,Homolog(S.Cerevisiae))(

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  • 产品厂商：BioAcademia
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简单介绍：
anti-NUF2antibody(NUF2,NDC80KinetochoreComplexComponent,Homolog(S.Cerevisiae))(C-Term)
详情介绍：
Immunogen Synthetic peptide CGGDSYAKIDEKTAELKRKMFKMS corresponding to the C-terminus region of human Nuf2
Specificity React with human and chicken Nuf2.
Cross-Reactivity (Details) Not tested in other species.
Characteristics Whole Antiserum
Purification Rabbit serum
Alternative Name Nuf2 (NUF2 Antibody Abstract)
Background Nuf2 is a component of kinetochore-associated Ndc80 complex, which serves to attach microtubules to the kinetochore. Nuf2 is a conserved protein from yeast to human. Disruption of the Nuf2 gene in Schizosaccharomyces pombe causes defect in chromosome segregation and in the spindle checkpoint. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. In human cells, Nuf2 specifically functions at kinetochores for stable microtubule attachment. Down regulation of the protein by RNA interference results in failure of the kinetochores to form attachments to the spindle microtubules. As a result, cells are blocked in the prometaphase stage with an active spindle checkpoint and undergo cell death. This antibody was prepared and tested by Prof. Tokuko Haraguchi at Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology
UniProt Q9BZD4
Pathways Maintenance of Protein Location
Application Notes 1. Western blotting: 500~5,000 fold dilution
2. Immunofluorescence staining: 200~1,000 fold dilution Methanol fixation is recommended for immunofluorescence staining.
Restrictions For Research Use only
Format Liquid
Buffer 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Immunofluorescence (IF) image for anti-NUF2, NDC80 Kinetochore Complex Component, Homolog (S. Cerevisiae) (NUF2) (C-Term) antibody (ABIN2452061) anti-NUF2, NDC80 Kinetochore Complex Component, Homolog (S. Cerevisiae) (NUF2) (C-Term) antibody
SDS-PAGE (SDS) image for anti-NUF2, NDC80 Kinetochore Complex Component, Homolog (S. Cerevisiae) (NUF2) (C-Term) antibody (ABIN2452061) anti-NUF2, NDC80 Kinetochore Complex Component, Homolog (S. Cerevisiae) (NUF2) (C-Term) antibody (Image 2)
Product cited in: DeLuca, Moree, Hickey, Kilmartin, Salmon: "hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells." in: The Journal of cell biology, Vol. 159, Issue 4, pp. 549-55, 2002 (PubMed).

Wigge, Kilmartin: "The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation." in: The Journal of cell biology, Vol. 152, Issue 2, pp. 349-60, 2001 (PubMed).

Nabetani, Koujin, Tsutsumi, Haraguchi, Hiraoka: "A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint." in: Chromosoma, Vol. 110, Issue 5, pp. 322-34, 2001 (PubMed).